Research Calculator
Peptide Reconstitution Calculator
Enter the peptide amount in the vial, the volume of bacteriostatic water you plan to add, and the target dose per injection. Calculations run in your browser — nothing is stored or transmitted.
Common amounts: 1 mL, 2 mL, 3 mL, 5 mL
Results
Concentration
2.500 mg/mL
Draw volume per dose
0.200 mL
On U-100 insulin syringe
20.0 units
Approx. doses per vial
10 doses
This calculator is provided for informational and research purposes only. It is not medical advice. Verify all calculations independently before use in a research protocol.
How peptide reconstitution works
Peptide reconstitution is the process of dissolving a lyophilized (freeze-dried) peptide using a diluent — typically bacteriostatic water containing 0.9% benzyl alcohol. The resulting solution must be drawn into a syringe at a known concentration so each dose can be measured accurately.
The core formula is:
concentration (mg/mL) = peptide amount (mg) ÷ diluent volume (mL)
On a standard U-100 insulin syringe, each full mL contains 100 units. So if you reconstitute a 5 mg vial with 2 mL of bacteriostatic water, the concentration is 2.5 mg/mL. Drawing 20 units (0.20 mL) yields 0.50 mg.
Frequently asked questions
How do I reconstitute a peptide vial with bacteriostatic water?
Divide the peptide amount in the vial by your target concentration to get the total reconstitution volume. For example, a 5 mg vial reconstituted with 2 mL of bacteriostatic water gives 2.5 mg/mL. A standard 100-unit insulin syringe holds 1 mL.
How long does reconstituted peptide last in bacteriostatic water?
Most reconstituted peptides remain stable for 28 days when stored at 2–8 °C in bacteriostatic water (0.9% benzyl alcohol preservative). Stability varies by peptide — consult a Certificate of Analysis or peer-reviewed reference for your specific compound.
What's the difference between bacteriostatic and sterile water for reconstitution?
Bacteriostatic water contains 0.9% benzyl alcohol, which inhibits microbial growth and allows multi-dose use for up to 28 days. Sterile water has no preservative and should be used immediately or discarded after single use.
Related Calculators
Need bacteriostatic water?
10 mL vials · per-lot CoA · same-day ship
For research and laboratory use only · Not for human or veterinary use
