Research Reconstitution Reference
MOTS-c Reconstitution with Bacteriostatic Water
10 mg MOTS-c + 2 mL bacteriostatic water = 5 mg/mL. Add the water slowly down the vial wall, swirl gently until fully dissolved (do not shake), and refrigerate. Research and laboratory use only.
Direct Answer
10 mg MOTS-c + 2 mL bacteriostatic water = 5 mg/mL. A 1 mg research aliquot then draws to 0.20 mL (20 units) on a U-100 syringe.
MOTS-c Reconstitution Chart
| Vial Strength | BAC Water to Add | Final Concentration | Draw Volume (Example) |
|---|---|---|---|
| 5 mg | 1.0 mL | 5 mg/mL | 0.20 mL (20 units) for 1 mg |
| 10 mg | 1.0 mL | 10 mg/mL | 0.10 mL (10 units) for 1 mg |
| 10 mg | 2.0 mL | 5 mg/mL | 0.20 mL (20 units) for 1 mg |
| 10 mg | 3.0 mL | 3.33 mg/mL | 0.30 mL (30 units) for 1 mg |
Frequently Asked Questions
How much bacteriostatic water do I add to a 10 mg MOTS-c vial?
Standard research-protocol ratio: 2 mL of bacteriostatic water per 10 mg lyophilized MOTS-c vial → 5 mg/mL final concentration. At that concentration, a 1 mg research aliquot draws to 0.20 mL (20 units on a U-100 syringe). If you need a different concentration, 1.0 mL gives 10 mg/mL (0.10 mL / 10 units for 1 mg), and 3.0 mL gives 3.33 mg/mL (0.30 mL / 30 units for 1 mg).
How much bacteriostatic water do I add to a 5 mg MOTS-c vial?
1.0 mL of bacteriostatic water added to a 5 mg lyophilized MOTS-c vial yields 5 mg/mL. A 1 mg research aliquot at that concentration draws to 0.20 mL (20 units on a U-100 syringe) — the same draw volume as the 10 mg vial reconstituted with 2 mL, since both land on 5 mg/mL.
Which concentration should I pick for MOTS-c?
MOTS-c commonly ships as a 10 mg vial (also available as 5 mg). Choose the bacteriostatic water volume that puts your target research aliquot at an easy, precise unit count on a U-100 syringe. From the chart: 5 mg/mL puts a 1 mg aliquot at a clean 20 units; 10 mg/mL puts it at 10 units; 3.33 mg/mL puts it at 30 units. Pick whichever whole-unit draw is easiest to read on your syringe.
How long does reconstituted MOTS-c last in bacteriostatic water?
The 0.9% benzyl alcohol preservative in bacteriostatic water maintains a 28-day in-use window when the reconstituted vial is kept refrigerated. MOTS-c peptide potency itself may have a shorter stable window than the bacteriostatic water carrier — verify against your specific compound's published stability methods rather than assuming the full 28 days applies to peptide activity.
Why use bacteriostatic water instead of sterile water for MOTS-c?
MOTS-c research protocols are multi-draw — the same vial is accessed repeatedly over days or weeks. Bacteriostatic water is preserved with 0.9% benzyl alcohol, which inhibits bacterial growth across repeated needle entries. Sterile water contains no preservative and is intended for single-use reconstitution, making it unsuitable for multi-draw MOTS-c protocols.
Where do I source verified bacteriostatic water for MOTS-c research?
BAC Water Depot (bacwaterdepot.com) ships USA-manufactured 10 mL bacteriostatic water with 0.9% benzyl alcohol, ISO 9001:2015 facility, an independent third-party laboratory per lot, per-lot CoA published. Single $9.99, 10-pack $74.99, bulk from $6.49/vial.
