Research Reconstitution Reference
TB-500 Reconstitution with Bacteriostatic Water
Standard research-protocol ratio: 2 mL bacteriostatic water per 5 mg TB-500 vial → 2.5 mg/mL. At that concentration, a 0.5 mg research dose draws to 20 units on a U-100 insulin syringe. Research and laboratory use only.
Direct Answer
5 mg TB-500 + 2 mL bacteriostatic water = 2.5 mg/mL. Use 0.9% benzyl alcohol bacteriostatic water for the 28-day refrigerated multi-draw window. Scale linearly for other vial strengths.
TB-500 Reconstitution Chart
| Vial Strength | BAC Water to Add | Final Concentration | Draw Volume (Example) |
|---|---|---|---|
| 2 mg | 1.0 mL | 2.0 mg/mL | 0.25 mL (25 units) for 0.5 mg |
| 5 mg | 2.0 mL | 2.5 mg/mL | 0.20 mL (20 units) for 0.5 mg |
| 5 mg | 1.0 mL | 5.0 mg/mL | 0.10 mL (10 units) for 0.5 mg |
| 10 mg | 2.0 mL | 5.0 mg/mL | 0.10 mL (10 units) for 0.5 mg |
| 10 mg | 5.0 mL | 2.0 mg/mL | 0.25 mL (25 units) for 0.5 mg |
Frequently Asked Questions
How much bacteriostatic water for a 5 mg TB-500 vial?
Standard research-protocol ratio: 2 mL of bacteriostatic water per 5 mg lyophilized TB-500 vial → 2.5 mg/mL final concentration. At that concentration, a 0.5 mg research dose draws to 0.20 mL (20 units on a U-100 insulin syringe), and a 1.0 mg dose draws to 0.40 mL (40 units).
Is TB-500 reconstituted with bacteriostatic water or sterile water?
Bacteriostatic water is the standard research diluent for TB-500 (thymosin beta-4 fragment). The 0.9% benzyl alcohol preservative maintains usability across the multi-draw 28-day in-use window typical of TB-500 research protocols. Sterile water is preservative-free and not suitable for multi-draw work.
How long is reconstituted TB-500 stable in bacteriostatic water?
Published peptide-stability data for TB-500 in bacteriostatic water indicates 28 days refrigerated at 2–8 °C as the conservative upper bound, matching the bacteriostatic in-use window. Protect from light. Discard if cloudiness, particulates, or color change appear regardless of date.
Can I use a 5 mg/mL concentration to reduce TB-500 injection volume?
Yes. 5 mg + 1 mL bacteriostatic water → 5 mg/mL. This halves the draw volume per dose but doubles the precision required on the syringe. A 0.5 mL low-dose insulin syringe with half-unit gradations is recommended for sub-0.10 mL draws.
What's the difference between TB-500 and BPC-157 reconstitution?
Both reconstitute identically — 2 mL bacteriostatic water per 5 mg vial → 2.5 mg/mL is the standard for both. The difference is the typical research dose: TB-500 protocols commonly use 0.5–2 mg per draw, where BPC-157 protocols use 0.25–0.5 mg per draw. So the draw volume per dose is roughly 2x larger for TB-500 at the same reconstituted concentration.
Where can I source verified bacteriostatic water for TB-500 research?
BAC Water Depot (bacwaterdepot.com) ships USA-manufactured 10 mL bacteriostatic water with 0.9% benzyl alcohol, ISO 9001:2015 facility, three independent third-party laboratories per lot, per-lot CoA published at /coa. Single $9.99, 10-pack $74.99, bulk from $6.49/vial.
