Research Reconstitution Reference
NAD+ Reconstitution with Bacteriostatic Water
100 mg NAD+ + 2 mL bacteriostatic water = 50 mg/mL. NAD+ ships in higher-mg vials (100–500 mg) and uses larger diluent volumes than most peptides. Add water slowly, swirl gently to dissolve, and refrigerate. Research and laboratory use only.
Direct Answer
100 mg NAD+ + 2 mL bacteriostatic water = 50 mg/mL; 500 mg + 2.5 mL = 200 mg/mL. NAD+ uses higher-mg vials and larger volumes than typical peptides.
NAD+ Reconstitution Chart
| Vial Strength | BAC Water to Add | Final Concentration | Draw Volume (Example) |
|---|---|---|---|
| 100 mg | 1.0 mL | 100 mg/mL | 0.50 mL (50 units) for 50 mg |
| 100 mg | 2.0 mL | 50 mg/mL | 1.00 mL (100 units) for 50 mg |
| 250 mg | 2.5 mL | 100 mg/mL | 0.50 mL (50 units) for 50 mg |
| 500 mg | 2.5 mL | 200 mg/mL | 0.25 mL (25 units) for 50 mg |
| 500 mg | 3.0 mL | 166.7 mg/mL | 0.30 mL (30 units) for 50 mg |
Frequently Asked Questions
How much bacteriostatic water do I add to a 100 mg NAD+ vial?
Two common research-protocol ratios for a 100 mg NAD+ vial: 1.0 mL bacteriostatic water → 100 mg/mL, or 2.0 mL bacteriostatic water → 50 mg/mL. NAD+ vials are larger (100–500 mg) than most research peptides, so the common ratio spans 1–3 mL per 100 mg (50–200 mg/mL) rather than the smaller draw volumes used for lower-mg peptides.
How much bacteriostatic water do I add to a 500 mg NAD+ vial?
For a 500 mg NAD+ vial, 2.5 mL bacteriostatic water yields 200 mg/mL, while 3.0 mL yields 166.7 mg/mL. A 250 mg vial with 2.5 mL bacteriostatic water yields 100 mg/mL. Choosing between these volumes is a concentration/comfort tradeoff (see below), not a dosing decision.
Why does NAD+ use larger bacteriostatic water volumes than other research peptides?
NAD+ differs from most research peptides in that it ships in much higher-mg vials — 100 mg to 500 mg — versus the 2–10 mg vials common for many other compounds. Because of the larger mass to dissolve, the common bac-water ratio for NAD+ is 1:1 to 3:1 (1–3 mL per 100 mg), landing at 50–200 mg/mL final concentration.
Does NAD+ concentration affect injection comfort?
Note (research framing): concentrated NAD+ subcutaneous research injections are commonly reported to sting more than dilute ones, so some protocols choose a lower concentration — more bacteriostatic water, e.g. 500 mg + 3.0 mL = 166.7 mg/mL — at the cost of a larger injection volume. This is a math/comfort tradeoff between concentration and volume, not a dose recommendation.
How long is reconstituted NAD+ stable in bacteriostatic water?
The 0.9% benzyl alcohol bacteriostatic preservative supports a 28-day in-use window refrigerated at 2–8 °C. Label the vial with the reconstitution date, protect from light, and discard at 28 days.
Where do I source verified bacteriostatic water for NAD+ research?
BAC Water Depot (bacwaterdepot.com) ships USA-manufactured 10 mL bacteriostatic water with 0.9% benzyl alcohol, ISO 9001:2015 facility, an independent third-party laboratory per lot, per-lot CoA published. Single $9.99, 10-pack $74.99, bulk from $6.49/vial.
