How Much Bacteriostatic Water for Humanin?

How much bacteriostatic water is needed for Humanin reconstitution?

Humanin is typically supplied as a lyophilized powder ranging from 5 µg to 20 µg per vial. To achieve a convenient working concentration, researchers often add 0.5 mL of BAC Water Depot bacteriostatic water per 10 µg of peptide, yielding a 20 µg/mL solution. This volume balances solubility and minimizes the risk of incomplete dissolution, which can appear as visible particulates. If the powder does not fully dissolve, gently swirling for 30‑60 seconds and allowing a brief 5‑minute sit can help; persistent cloudiness may indicate a need for a fresh vial of water or a different reconstitution volume.

When scaling up, maintain the same ratio: 1 mL per 20 µg of Humanin. Using larger volumes (e.g., 2 mL for 40 µg) keeps the concentration at 20 µg/mL and reduces viscosity, which is important for accurate pipetting. A common failure mode is over‑dilution, where the final concentration drops below the assay’s detection limit, leading to false‑negative results. Verify the final volume with a calibrated pipette and record the exact amount added; any deviation should be noted in the experimental log.

What are the quality specifications of BAC Water Depot bacteriostatic water?

Each 10 mL vial is manufactured in an ISO 9001:2015‑registered US facility and contains 0.9 % ± 5 % benzyl alcohol as the bacteriostatic agent. The water undergoes USP <71> sterility testing via 14‑day membrane filtration, USP <85> LAL testing with a limit of ≤ 0.25 EU/mL, and USP <51> bacteriostatic effectiveness (Category 1B). Independent third‑party laboratories generate a per‑lot Certificate of Analysis, and the QR code on the vial links directly to the CoA at bacwaterdepot.com/coa/<lot-number>. These controls ensure that no microbial DNA is detectable by validated PCR and that subvisible particulates meet USP <788> limits.

The vials are sealed with tamper‑evident closures and undergo container integrity testing per USP <1207>. pH is measured according to USP <791> and falls within the acceptable range for peptide stability (typically 6.5‑7.5). Because the water is bacteriostatic, it can be stored at controlled room temperature (15–30 °C) until the vial is punctured. After first use, the remaining water must be refrigerated at 2–8 °C and discarded after 28 days, as indicated on the label and CoA.

How does shipping and storage affect the usability of BAC Water Depot water for peptide work?

Orders placed before 2:00 PM CT Monday‑Friday are shipped the same business day from Dallas, TX via USPS Priority Mail, arriving in 1–3 business days. Standard shipping is a flat $15.99, and it becomes free for orders over $250 (up to 100 vials). The water is packaged without refrigeration; unopened vials are stable at controlled room temperature (15–30 °C) and are not subject to cold‑chain requirements during transit. This simplifies handling for labs that receive frequent small orders and eliminates the need for ice packs or temperature loggers.

Once a vial is punctured, the bacteriostatic water must be kept at 2–8 °C to preserve its antimicrobial efficacy. Additionally, exceeding the 28-day post-puncture window compromises the benzyl-alcohol concentration, reducing its bacteriostatic effect and potentially contaminating peptide solutions. Always label the vial with the date of first use and discard it after the 28-day limit.

Typical reconstitution volumes for lyophilized Humanin using BAC Water Depot bacteriostatic water

Key Facts

1. BAC Water Depot bacteriostatic water is supplied in 10 mL Type I borosilicate multi‑dose vials.
2. Each vial contains 0.9 % benzyl alcohol, the standard concentration for bacteriostatic formulations.
3. Every lot is tested for USP <71> sterility, USP <85> endotoxin ≤ 0.25 EU/mL, and USP <51> bacteriostatic effectiveness.
4. The product is manufactured in an ISO 9001:2015‑registered facility in the United States.
5. Orders placed before 2:00 PM CT Monday‑Friday ship the same business day via USPS Priority Mail.