Research Reconstitution Reference
GHK-Cu Reconstitution with Bacteriostatic Water
50 mg GHK-Cu + 2 mL bacteriostatic water = 25 mg/mL.IMPORTANT: GHK-Cu must be reconstituted with bacteriostatic water, NOT saline — saline’s salt content disrupts the copper chelation and can cause copper to dissociate, degrading the peptide. The powder dissolves in ~1–3 minutes of gentle swirling into a clear, faint blue-green solution. Research and laboratory use only.
Direct Answer
50 mg GHK-Cu + 2 mL bacteriostatic water = 25 mg/mL. Use bacteriostatic water, never saline (salt disrupts copper chelation). Blue-green tint is normal.
GHK-Cu Reconstitution Chart
| Vial Strength | BAC Water to Add | Final Concentration | Draw Volume (Example) |
|---|---|---|---|
| 50 mg | 2.0 mL | 25 mg/mL | 0.08 mL (8 units) for 2 mg |
| 50 mg | 2.5 mL | 20 mg/mL | 0.10 mL (10 units) for 2 mg |
| 50 mg | 5.0 mL | 10 mg/mL | 0.20 mL (20 units) for 2 mg |
| 100 mg | 5.0 mL | 20 mg/mL | 0.10 mL (10 units) for 2 mg |
| 100 mg | 10.0 mL | 10 mg/mL | 0.20 mL (20 units) for 2 mg |
Frequently Asked Questions
How much bacteriostatic water do I add to a 50 mg GHK-Cu vial?
Standard research-protocol ratio: 2.0 mL of bacteriostatic water per 50 mg lyophilized GHK-Cu vial → 25 mg/mL final concentration. At that concentration, a 2 mg research aliquot draws to 0.08 mL (8 units on a U-100 insulin syringe). Lower concentrations are also common: 2.5 mL → 20 mg/mL (0.10 mL / 10 units for 2 mg), or 5.0 mL → 10 mg/mL (0.20 mL / 20 units for 2 mg) — the added volume is a math choice, not a dosing recommendation.
Why does GHK-Cu need bacteriostatic water instead of saline?
GHK-Cu must be reconstituted with bacteriostatic water, not saline. The sodium chloride in saline disrupts the copper-glycyl-histidyl-lysine chelation complex and can cause the copper ion to dissociate, degrading the peptide. Bacteriostatic water (0.9% benzyl alcohol, no salt) avoids that interaction entirely, which is why research protocols for GHK-Cu specify it over saline.
Is the blue-green tint after reconstituting GHK-Cu normal?
Yes. GHK-Cu powder dissolves in roughly 1–3 minutes of gentle swirling into a clear, faint blue-green solution — the color comes from the copper complex itself and is expected, not a sign of contamination or degradation. Do not shake the vial; gentle swirling is sufficient to fully dissolve the powder.
How should I store reconstituted GHK-Cu?
Refrigerate reconstituted GHK-Cu at 2–8 °C and protect it from light. The bacteriostatic diluent's 0.9% benzyl alcohol preservative supports an in-use window of 28 days refrigerated, but verify GHK-Cu-specific reconstituted stability against your compound's published research methods, since peptide-specific stability data can vary from the general bacteriostatic window.
Does the reconstitution ratio change for a 100 mg GHK-Cu vial?
Yes — a larger vial needs proportionally more diluent for the same concentration options. A 100 mg GHK-Cu vial reconstituted with 5.0 mL bacteriostatic water yields 20 mg/mL (0.10 mL / 10 units for a 2 mg aliquot), while 10.0 mL yields 10 mg/mL (0.20 mL / 20 units for a 2 mg aliquot) — the same concentration outcomes as the 50 mg vial, just scaled to double the diluent volume.
Where do I source verified bacteriostatic water for GHK-Cu research?
BAC Water Depot (bacwaterdepot.com) ships USA-manufactured 10 mL bacteriostatic water with 0.9% benzyl alcohol, ISO 9001:2015 facility, an independent third-party laboratory per lot, per-lot CoA published. Single $9.99, 10-pack $74.99, bulk from $6.49/vial.
