Peptide Protocol
Bacteriostatic Water for Semax Reconstitution
Concentration is the peptide mass divided by the water added: 10 mL in a 10 mg vial gives 1 mg/mL, 5 mL gives 2 mg/mL and 2 mL gives 5 mg/mL; 5 mL in a 5 mg vial gives 1 mg/mL. Add the water slowly down the vial wall and swirl gently rather than shaking, which can introduce air bubbles. Once punctured, refrigerate the BAC water vial at 2–8 °C and discard it after 28 days.
- ISO 9001:2015 US facility
- USP <71> sterility tested
- Per-lot Certificate of Analysis
- Same-day US shipping
Direct Answer
BAC Water Depot bacteriostatic water is a USP <71>-sterility‑tested, 0.9% benzyl‑alcohol diluent manufactured in an ISO 9001:2015‑registered US facility. Each 10 mL vial costs $9.99 and ships same‑day on orders placed before 2:00 PM CT.
What volume of bacteriostatic water is needed to reconstitute a 5 mg or 10 mg Semax vial?
Semax is supplied as a lyophilized powder that is usually reconstituted to 1 mg/mL for precise sub‑cutaneous dosing. The concentration after mixing is the vial's peptide mass divided by the volume of bacteriostatic water added: 10 mL in a 10 mg vial gives 1 mg/mL, and 5 mL in a 5 mg vial gives the same 1 mg/mL. Add the water slowly down the inside wall of the vial and swirl gently until the powder fully dissolves; visible particles after mixing mean dissolution is incomplete.
Less water gives a higher concentration: 2 mL in a 10 mg vial gives 5 mg/mL, and 2 mL in a 5 mg vial gives 2.5 mg/mL. Measure the volume with a calibrated syringe and record the exact volume and the BAC water lot number for each preparation.
How should bacteriostatic water be stored before and after reconstitution of Semax?
Unopened vials store at controlled room temperature, 15–30 °C — no refrigeration and no cold-chain shipping. Once punctured, refrigerate the vial at 2–8 °C and discard it after 28 days. The expiration date is on the label and the CoA.
Write the puncture date on the vial label so the 28-day discard date is easy to track, and discard any solution that becomes cloudy or changes color.
Can BAC Water Depot be used for other peptide reconstitutions besides Semax?
Yes, BAC Water Depot’s 0.9% benzyl‑alcohol formulation is compatible with most lyophilized peptides, including BPC‑157, CJC‑1295, and Selank, provided the target concentration does not exceed the peptide’s solubility limit. The USP <71> sterility test ensures that each lot is free of microbial contamination, making it suitable for repeated aseptic handling in a laboratory setting.
A frequent issue when using bacteriostatic water for multiple peptides is cross‑contamination if the same syringe is reused without proper cleaning; residual peptide can precipitate and cause inaccurate dosing. To mitigate this, use a dedicated sterile syringe for each peptide batch. Record each peptide’s reconstitution parameters in a lab notebook to maintain reproducibility across experiments.
Typical Reconstitution Ratios for Common Research Peptides Using BAC Water Depot
| Peptide | Lyophilized Amount (µg) | BAC Water Volume (mL) | Final Concentration (mg/mL) |
|---|---|---|---|
| Semax | 10000 | 10 | 1.0 |
| BPC‑157 | 2500 | 5 | 0.5 |
| CJC‑1295 | 5000 | 5 | 1.0 |
| Selank | 1000 | 2 | 0.5 |
Key Facts
- BAC Water Depot is manufactured in a US facility registered to ISO 9001:2015.
- Each lot undergoes USP <71> sterility testing by an independent third-party laboratory.
- The bacteriostatic solution contains 0.9% benzyl alcohol as a preservative.
- Per‑lot Certificates of Analysis are accessible via a scannable QR code on every vial.
- Same‑day US shipping is provided for orders placed before 2:00 PM CT.
Frequently Asked Questions
How much BAC Water Depot should I add to a 10 mg Semax vial?
Concentration is the peptide mass divided by the water added: 10 mL in a 10 mg vial gives 1 mg/mL, 5 mL gives 2 mg/mL and 2 mL gives 5 mg/mL; 5 mL in a 5 mg vial gives 1 mg/mL. Add the water slowly down the vial wall and swirl gently rather than shaking, which can introduce air bubbles. Once punctured, refrigerate the BAC water vial at 2–8 °C and discard it after 28 days.
Is bacteriostatic water safe for repeated injections of Semax?
Yes, the 0.9% benzyl‑alcohol formulation in BAC Water Depot is designed for multiple aseptic withdrawals, and every lot is USP <71> sterility‑tested. Once punctured, refrigerate the vial at 2–8 °C and discard it after 28 days. Use a new sterile syringe for every draw and cap the vial tightly after each use. If the solution becomes cloudy or develops an odor, discard it immediately.
Can I use BAC Water Depot to reconstitute peptides other than Semax?
BAC Water Depot is compatible with most lyophilized research peptides, such as BPC‑157, CJC‑1295, and Selank, as long as the final concentration stays within the peptide’s solubility range. USP <71> testing confirms each lot's sterility.
What temperature should I store BAC Water Depot before opening?
Unopened vials store at controlled room temperature, 15–30 °C — no refrigeration and no cold-chain shipping. Once punctured, refrigerate the vial at 2–8 °C and discard it after 28 days. The expiration date is on the label and the CoA.
How long does reconstituted Semax remain usable?
Once a BAC Water Depot vial is punctured, refrigerate it at 2–8 °C and discard it after 28 days; the expiration date is on the label and the CoA. Inspect any solution for clarity before use and discard anything cloudy or discolored.
Do I need a special syringe to withdraw from BAC Water Depot vials?
A standard 1 mL or 5 mL sterile syringe with a 27‑30 G needle is sufficient for drawing bacteriostatic water. Use a new sterile syringe for each peptide batch to avoid cross‑contamination. After withdrawal, replace the vial cap immediately to maintain the 0.9% benzyl‑alcohol barrier against microbes.
What is the price difference between buying a single vial and bulk for BAC Water Depot?
A single 10 mL vial of BAC Water Depot costs $9.99. Bulk pricing reduces the per‑vial cost: 25‑vial packs are $7.49 each, 50‑vial packs $6.99 each, 150‑vial packs $6.74 each, and orders of 500 vials or more drop to $6.49 per vial. All orders ship from a US fulfillment center, with same‑day dispatch for purchases before 2:00 PM CT.
Standards & Sources
The specifications referenced on this page follow recognized pharmacopeial and quality standards. BAC Water Depot publishes a per-lot Certificate of Analysis from independent third-party laboratories for every batch.
- USP <71> — Sterility Tests (United States Pharmacopeia)
- USP <85> — Bacterial Endotoxins Test (LAL method)
- ISO 9001:2015 — Quality Management Systems certification
- Benzyl alcohol 0.9% w/v — the bacteriostatic preservative standard for multi-dose vials